Plasmid_Backbone

Part:BBa_K4054011

Designed by: Xinyan Han   Group: iGEM21_XJTLU-CHINA   (2021-10-01)


pCRISPR

This is an plasmid with cleage site for crRNA, which could be used as dua-plasmid CRISPR system with pCas9 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2348
    Illegal EcoRI site found at 2622
    Illegal SpeI site found at 1138
    Illegal PstI site found at 389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2348
    Illegal EcoRI site found at 2622
    Illegal SpeI site found at 1138
    Illegal PstI site found at 389
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2348
    Illegal EcoRI site found at 2622
    Illegal BglII site found at 176
    Illegal BamHI site found at 2082
    Illegal BamHI site found at 2356
    Illegal XhoI site found at 2702
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2348
    Illegal EcoRI site found at 2622
    Illegal SpeI site found at 1138
    Illegal PstI site found at 389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2348
    Illegal EcoRI site found at 2622
    Illegal SpeI site found at 1138
    Illegal PstI site found at 389
    Illegal NgoMIV site found at 840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2280
    Illegal BsaI site found at 2554
    Illegal BsaI.rc site found at 2257
    Illegal BsaI.rc site found at 2531
    Illegal SapI.rc site found at 689
    Illegal SapI.rc site found at 899


[edit]
Categories
Parameters
None