Part:BBa_K4054010

pCas9

This is an plasmid with Cas9 coding gene, which could be used as dua-plasmid CRISPR system with pCRISPR plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 3564
  Illegal XbaI site found at 1425
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 3564
  Illegal NheI site found at 583
  Illegal NheI site found at 3323
  Illegal NheI site found at 6805
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 3564
  Illegal BamHI site found at 5602
  Illegal BamHI site found at 6951
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 3564
  Illegal XbaI site found at 1425
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1
  Illegal EcoRI site found at 3564
  Illegal XbaI site found at 1425
  Illegal NgoMIV site found at 6977
  Illegal NgoMIV site found at 7345
  Illegal NgoMIV site found at 7505
  Illegal NgoMIV site found at 7859
  Illegal NgoMIV site found at 8439
  Illegal AgeI site found at 669
  Illegal AgeI site found at 993
  Illegal AgeI site found at 8263
  Illegal AgeI site found at 8723
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 6544
  Illegal BsaI.rc site found at 6521