Primer
pTF_tal_re

Part:BBa_K4047004

Designed by: An Nguyen, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-07)


primerTF_tal-reverse

Rev primer for transadolase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the reverse primer that pairs with the forward primer BBa_K4047005. Together they amplify and allow insertion of transaldolase and fructose-1,6-bisphosphatase into pGEM-tal+ fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.

This primer does not contain any BioBrick incompatible sequences, making it a very useful option for teams that want to amplify transaldolase from the wild type genome.

[edit]
Categories
//primer/part
Parameters
None