Composite

Part:BBa_K3970025

Designed by: Xuan Gong   Group: iGEM21_ECUST_China   (2021-10-01)


pGAP-PcyA-tADH1

Usage and Biology

Gene PcyA encodes ferredoxin oxidoreductase, which catalyzes the four-electron reduction of biliverdin IX-alpha (2-electron reduction at both the A and D rings) and then form (2R,3Z)-phycocyanobilin:

biliverdin IX-alpha + 4 H+ + 4 reduced [2Fe-2S]-[ferredoxin] = biliverdin IX-alpha + 4 oxidized [2Fe-2S]-[ferredoxin]

Construction

0025-1.png

Experiment results

( note that how we recycle the GAP promoter and ADH1 terminator has been shown on the last parts. And the sequence pcyA has already been synthesized on the plasmid pTDH3 so we don’t have to recycle it.)

Sequence

tcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaaagatctgatatggatcgaattagatctcgccaccatgccatgtagaaagttgtgtgatgttgaattaggttcagtcttgatgtctttgagacaacaacaacacccattaatccaaagactggctgatagaatcgaacaaatctggcaaacccacttgcaattagccccatacgaattgccagcagacttgggttacgtcgaaggcaagttggagggtgaaagattggttattgaaaaccattgttaccaagctcctccattcagaaagcttcacctagaattggctcgtgttggtaatactttggatattctacattgcgttatgttcccacaaccaacttacgacttgccaatgtttggttgtgacttggtcggtggtagaggtcaaatctctgctgccattgtcgatttatctccagttaccggtgaattgccaccagcttacaccactgctttgaacgctttaccaaagatcgccttctcccaaaccagagaattgccaccatggggttacatcttctctcctttctgtttgttcattagaccacaaggtgcttccgaagaacagcaattcttggatcgtatcggtgactacttggccttgcactgtcaattgtctcaacaatccaccccattgttggacccacaaccagttattgctggtcaacaacaatactgtcaacaacaacaacaaaacgacaaaactcgtagagttttggaaaaggctttcggtcacgactgggctgaaagatatatgactactgtcttgtttgatgctccatgagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccgg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None