Part:BBa_K3970010

PcyA

Usage and Biology

Gene PcyA encodes ferredoxin oxidoreductase, which catalyzes the four-electron reduction of biliverdin IX-alpha (2-electron reduction at both the A and D rings) and then form (2R,3Z)-phycocyanobilin:
biliverdin IX-alpha + 4 H+ + 4 reduced [2Fe-2S]-[ferredoxin] = biliverdin IX-alpha + 4 oxidized [2Fe-2S]-[ferredoxin]

Optimized

Original Sequence (Original Sequence Length: 750bp,GC%: 52.4):

ATGCCCTGTCGCAAGTTGTGCGACGTTGAGCTAGGATCAGTACTTATGTCTCTCCGTCAGCAACAACATCCCCTCATTCAACGCTTGGCCGATCGCATCGAGCAGATTTGGCAGACTC

ACCTGCAGCTTGCTCCTTACGAGTTACCCGCAGACCTTGGCTATGTTGAAGGCAAACTGGAGGGAGAGCGCCTCGTCATTGAAAACCACTGCTACCAAGCTCCTCCCTTTCGCAAACT

GCATTTAGAATTGGCACGGGTGGGGAACACCCTCGATATTTTGCACTGCGTGATGTTTCCGCAGCCAACCTATGATCTGCCCATGTTTGGCTGTGACTTAGTGGGAGGGCGTGGGCA

AATTAGTGCTGCCATTGTTGATCTGTCTCCTGTGACTGGGGAGCTACCCCCTGCCTACACTACTGCCTTGAATGCACTGCCTAAGATAGCCTTTAGCCAGACGCGGGAGCTGCCGCCA

TGGGGCTATATTTTTTCGCCATTTTGCCTTTTTATTCGCCCCCAAGGTGCTAGCGAAGAGCAGCAATTTTTAGATCGCATTGGCGACTATCTAGCGCTTCACTGCCAGCTAAGCCAACA

GAGCACCCCCCTCCTCGATCCCCAGCCAGTGATTGCTGGTCAACAGCAATATTGCCAGCAACAACAGCAAAACGACAAAACACGGCGAGTCCTTGAAAAAGCCTTTGGTCACGACTG

GGCGGAGCGGTATATGACCACTGTACTGTTTGATGCACCATAG

Optimized Sequence(Optimized Sequence Length:750bp,GC%:43.73):

ATGCCATGTAGAAAGTTGTGTGATGTTGAATTAGGTTCAGTCTTGATGTCTTTGAGACAACAACAACACCCATTAATCCAAAGACTGGCTGATAGAATCGAACAAATCTGGCAAACCCA

CTTGCAATTAGCCCCATACGAATTGCCAGCAGACTTGGGTTACGTCGAAGGCAAGTTGGAGGGTGAAAGATTGGTTATTGAAAACCATTGTTACCAAGCTCCTCCATTCAGAAAGCTT

CACCTAGAATTGGCTCGTGTTGGTAATACTTTGGATATTCTACATTGCGTTATGTTCCCACAACCAACTTACGACTTGCCAATGTTTGGTTGTGACTTGGTCGGTGGTAGAGGTCAAAT

CTCTGCTGCCATTGTCGATTTATCTCCAGTTACCGGTGAATTGCCACCAGCTTACACCACTGCTTTGAACGCTTTACCAAAGATCGCCTTCTCCCAAACCAGAGAATTGCCACCATGGG

GTTACATCTTCTCTCCTTTCTGTTTGTTCATTAGACCACAAGGTGCTTCCGAAGAACAGCAATTCTTGGATCGTATCGGTGACTACTTGGCCTTGCACTGTCAATTGTCTCAACAATCCA

CCCCATTGTTGGACCCACAACCAGTTATTGCTGGTCAACAACAATACTGTCAACAACAACAACAAAACGACAAAACTCGTAGAGTTTTGGAAAAGGCTTTCGGTCACGACTGGGCTGA

AAGATATATGACTACTGTCTTGTTTGATGCTCCATGA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]