Part:BBa_K3970008

CpcE

Usage and Biology

CpcE encodes phycocyanin alpha Phycocyanobilin lyase which is one part of the Phycocyanobilin lyase catalyzing alpha phycocyanin and 3Z-phycocyanobilin.Phycocyanobilin lyase is required for the chromophorylation of α-phycocyanin to form holo-α-subunit.

Optimized

Original Sequence (Original Sequence Length: 663bp,GC%: 55.66):

ATGCTATCCCTTGAGCAGCTTGCCACCCAGCTTGAAAGTCCGAATTCCCGCGATCGCCTCCTTGCCTTGGCGGCGCTGCGGGATGTGCCCCCGCCTGAGGCTGTCCCGTTAATCAAA

AAAGTACTGTGGGATGATAATTTACAAATCCGCTCCATGGCTGTGTTTGCCTTAGGGGTTAAGCCCACGCCAGAGTGCTTTGACATTTTAGTGCAACTCCTCGAAACAGAACCCGACT

ACGGCATTCGCGCTGATGCTGCCGGAGCATTAGGGTATCTGCAAGATCAGCGGGCATTTGCCCCCCTCGTGCGTGCCTTTTACGAAGACACCGACTGGTTAGTGCGCTTTAGTGCAG

CAGTTTCCCTGGGCAACCTGAAGGATGTGCGGGCGGTTGAGGTGCTGCGCTCAGCCTTAAGCAGTAACGAGGTGGTGATGCTCCAAGGCGCGATCGCGGCGTTGGGGGAAATTGG

TGACCCCAGCATTGTTGAAGAGATTTTACCCTTTGTGACTGCCGAGGACTGGCTCATTCGCCAGCGACTTGCGGAAGCCTTGGGCAACCTCCCCAGCCCGAAAACTCAGTCAGCCTT

AGAGTATTTGGCCAAGGACGAGCATCCCCACGTTGCAGCAGCAGCAGAAATTTCTTTACAACGCCTTGCCCAGCGATAG

Optimized Sequence(Optimized Sequence Length:663bp,GC%:45.25):

ATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAA

GGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACG

GTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGT

TTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCAT

CCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTT

GGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGA

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]