Coding

Part:BBa_K3946055

Designed by: Baifern Wongthanaroj   Group: iGEM21_Edinburgh   (2021-10-21)


L2NC+linker+cex

A truncated version of the L2 ribosomal protein from E. coli, designed for fusion to C-terminal of cex using JUMP assembly but with the addition of a short linker (GSEGKSSGSGSESKST). This tag contains just the N and C-terminal regions of L2 which were shown to have silica binding capacity in previous experiments, therefore allowing the use of a smaller tag without compromising on binding affinity. This tag facilitates immobilisation to silica surfaces, enabling enzyme immobilisation or purification using silica-based spin columns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1714
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None