Coding

Part:BBa_K3946050

Designed by: Fiona Bunn   Group: iGEM21_Edinburgh   (2021-10-21)


Car9 silica tag+sfGFP

A short silica-binding tag to add to the C-terminal of sfGFP gene using JUMP assembly, including a short glycine-rich linker (GSGGGS). The tag facilitates immobilisation to silica surfaces, enabling enzyme immobilisation or purification using silica-based spin columns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


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Categories
Parameters
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