Composite
CAI-1 dete

Part:BBa_K3914105

Designed by: Liqing Yu   Group: iGEM21_SUSTech_Shenzhen   (2021-10-01)


the CAI-1 detector engineering sequences

This part is used to detect V.cholerae’s autoinducer CAI-1 by using three cascading phosphorylated proteins. With the inhibition caused by a series of proteins, we can finally get an inducing promotor tpQrr with high selectivity toward CAI-1 concentration. With this part, we can use a promotor controlled by the cell density of V.cholerae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 779
    Illegal PstI site found at 2568
    Illegal PstI site found at 3870
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1584
    Illegal NheI site found at 1607
    Illegal NheI site found at 2136
    Illegal NheI site found at 2159
    Illegal PstI site found at 779
    Illegal PstI site found at 2568
    Illegal PstI site found at 3870
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 779
    Illegal PstI site found at 2568
    Illegal PstI site found at 3870
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 779
    Illegal PstI site found at 2568
    Illegal PstI site found at 3870
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 831

Profile

Name: the CAI-1 detector engineering sequences
Base Pairs: 4421bp
Origin: Vibrio cholerae, V. cholerae
Properties: gene


references

Holowko, M. B.; Wang, H.; Jayaraman, P.; Poh, C. L., Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth Biol 2016, 5 (11), 1275-1283.

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