Part:BBa_K3914038

gadR Cl−-dependent positive regulator with rrnBT1T2 terminator

Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis and regulated by chloride-dependent positive regulator upstream gene gadR and its promoter pgadR. This regulator is similar as BBa_ K559011, However, to reduce stable hairpin structure for gene synthesis, we changed the terminator of gadR into a double terminator of rrnb T1T2.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 377
  Illegal EcoRI site found at 407
  Illegal EcoRI site found at 914
  Illegal EcoRI site found at 938
  Illegal XbaI site found at 1340
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 377
  Illegal EcoRI site found at 407
  Illegal EcoRI site found at 914
  Illegal EcoRI site found at 938
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 377
  Illegal EcoRI site found at 407
  Illegal EcoRI site found at 914
  Illegal EcoRI site found at 938
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 377
  Illegal EcoRI site found at 407
  Illegal EcoRI site found at 914
  Illegal EcoRI site found at 938
  Illegal XbaI site found at 1340
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 377
  Illegal EcoRI site found at 407
  Illegal EcoRI site found at 914
  Illegal EcoRI site found at 938
  Illegal XbaI site found at 1340
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1070
  

Profile

Name: chloride-dependent positive regulator with rrnBT1T2 terminator[1]
Base Pairs: 556bp
Origin: Lactococcus lactis subsp. cremoris MG1363
Properties: gene

reference

[1] Sanders, J. W., et al. (1998). "A chloride-inducible acid resistance mechanism in Lactococcus lactis and its regulation." Molecular Microbiology 27(2): 299-310.