Coding
ORFA

Part:BBa_K3914037

Designed by: Cai PeiJing   Group: iGEM21_SUSTech_Shenzhen   (2021-10-01)


open reading frame

OrfA is similar to an 11.2-kDa hypothetical protein of the rfaH-fre intergenic region of the E. coli chromosome. Therefore it may be useful for the expression of AcmA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Profile

Name: orfA[1][2]
Base Pairs: 285bp
Origin: Lactococcus lactis subsp. cremoris MG1363
Properties: gene


references

[1] Buist, G., et al. (1995). "Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation." Journal of Bacteriology 177(6): 1554-1563.

[2] Inagaki, N., et al. (2009). "Molecular properties of the glucosaminidase AcmA from Lactococcus lactis MG1363: Mutational and biochemical analyses." Gene 447(2): 61-71.

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