Coding
CqsS

Part:BBa_K3914013

Designed by: Liqing Yu   Group: iGEM21_SUSTech_Shenzhen   (2021-10-01)


the detector protein of vibrio cholerae quorum sensing signal CAI-1

CqsS gene codes a transmembrane receptor that will detect the concentration of CAI-1 in the cell outer environment. The expression level of CqsS will cause different phosphorylation levels of LuxU, which is a downstream protein in the cascading system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 379
    Illegal PstI site found at 1681
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 379
    Illegal PstI site found at 1681
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 379
    Illegal PstI site found at 1681
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 379
    Illegal PstI site found at 1681
  • 1000
    COMPATIBLE WITH RFC[1000]

Profile

Name: CqsS
Base Pairs: 2058bp
Origin: Vibrio cholerae, V. cholerae
Properties: gene


references

Holowko, M. B.; Wang, H.; Jayaraman, P.; Poh, C. L., Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth Biol 2016, 5 (11), 1275-1283.

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