Part:BBa_K3914006

Esa box of EsaR protein

EsaR from Pantoea stewartii subsp. stewartii is a quorum-sensing (QS) regulator that binds to DNA as an apoprotein and releases the DNA when it binds to its cognate signal molecule, 3-oxohexanoyl-homoserine lactone (3OC6HSL). In the absence of 3OC6HSL, EsaR binds to DNA and can act as either an activator or a repressor of transcription^[1][2].In this experiment, researchers use the Esa box to sense the EsaR for the detection of the Vibiro choleare’s QS signal, AHL^[3].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

reference

[1] Shong, J.; Huang, Y. M.; Bystroff, C.; Collins, C. H., Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol 2013, 8 (4), 789-95.

[2] Minogue, T. D.; Trebra, M. W.-v.; Bernhard, F.; Bodman, S. B. v., The autoregulatory role of EsaR, a quorum-sensing regulator in Pantoea stewartii ssp. stewartii: evidence for a repressor function. 2002, 44 (6), 1625-1635.

[3] Shong, J.; Collins, C. H., Quorum sensing-modulated AND-gate promoters control gene expression in response to a combination of endogenous and exogenous signals. ACS Synth Biol 2014, 3 (4), 238-46.