Coding

Part:BBa_K3868059

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-15)


sgRNA-CM

Gene for pTarget plasmids which can target RBS sequence of the T7 RNAP. This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pTarget+donnr plasmids.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 139
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 139
    Illegal BglII site found at 169
    Illegal XhoI site found at 193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


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