Part:BBa_K3866011

MazF toxin GB compatible with B3-B5

MazF toxin is used in the toxin-antitoxin system with mazE anti-toxin.This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity

Fig.2:MazF toxin into PUPD2 with GB compatible ends.

Usage and Biology

The MazEF system is an extensively studied type II TA module. MazF homologs are well known as ribosome-independent toxins and have sequence-specific endoribonucleases that can cleave mRNA sequences in a ribosome-independent manner.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.

Fig.1:The overhangs of this part in the GoldenBraid Grammar

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

• Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107