Composite

Part:BBa_K3861020

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)


PlldR-GFP(Harmonized for Salmonella Typhimurium)

Salmonella Typhimurium codon harmonized GFP expressed by the lactate-inducible PlldR promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 1065
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal NheI site found at 106
    Illegal NheI site found at 129
    Illegal SpeI site found at 1066
    Illegal PstI site found at 1080
    Illegal NotI site found at 13
    Illegal NotI site found at 1073
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 1066
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 1056
    Illegal XbaI site found at 22
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 842


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Categories
Parameters
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