Part:BBa_K3855607

Levanase sacC Expression casette for L. plantarum with an inducable Promotor and Secretion Signal

Expression cassettes for the expression and secretion of Levanase sacC in Lactobacillus. This enzyme facilitates the hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues. It features an inducible promotor, which can be activated using Sakacin A Inducing Peptide/Sakacin P Inducing Peptide (SapIP/SppIP) (Sørvig et al. 2003 FEMS Microbiology Letters 229, 119-126).

This was necessary for us as we assembled the part using the type 2 s system in E.coli and the enzyme proved to be toxic to the host. In the final host, Lactobacillus plantarum, we were able to induce the expression and demonstrate the secretion of the enzyme using a slot plot. This was possible as we added an N terminal his-tag to the enzyme.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1790
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]