Composite

Part:BBa_K3855601

Designed by: Sebastian Bayer   Group: iGEM21_BOKU-Vienna   (2021-10-15)


Endi Inulase Expression casette for L. plantarum with an inducible Promotor and Secretion Signal

Facilitates endohydrolysis of (2->1)-beta-D-fructosidic linkages in inulin. Expression casette for L.plantarum with an inducible promotor. Enzyme features a His-Tag and a secretion signal.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 200
    Illegal SpeI site found at 455
    Illegal SpeI site found at 557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1463
    Illegal SpeI site found at 200
    Illegal SpeI site found at 455
    Illegal SpeI site found at 557
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1580
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 200
    Illegal SpeI site found at 455
    Illegal SpeI site found at 557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 200
    Illegal SpeI site found at 455
    Illegal SpeI site found at 557
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None