Composite

Part:BBa_K3841071

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-10-17)


Expression cassette of pMMO alpha subunit with constitutive promoter

BBa_K3841070 is an expression cassette designed for Komagataella phaffii GS115. The PmoB protein and TEF terminator are codon-optimized for K. phaffii under the control of the TEF1 promoter.

Folding of PmoB (BBa_K3841010) with signal peptide (BBa_K3841036) and PmoB (BBa_K3841010) simulated using AlphaFold [1]. This expression cassette has follows the pink structure.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1556
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 737
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1556
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Cramer, Patrick. “AlphaFold2 And the Future of Structural Biology.†Nature Structural and Molecular Biology, vol. 28, no. 9, Nature Research, 2021, pp. 704–05, doi:10.1038/s41594-021-00650-1.

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