Part:BBa_K3841036

Plasma membrane signal peptide for Komagataella phaffii

In Saccharomyces cerevisiae the GAS family encodes GPI-anchored proteins required for cell wall or spore wall assembly [1]. BBa K3841036 is a predicted signal peptide of the Gas3 (UniProt accession: F2QQJ2), a putative 1,3-beta-glucanosyltransferase located in the plasma membrane of in Komagataella phaffii GS115 (previously denoted Pichia pastoris GS115) [2]. The signal peptide was further investigated using Deeploc [3] to support the predicted localization in the plasma membrane of the protein.

Deeploc tree with predicted location for the Gas3 habouring the signal peptide of this part.

Usage
The signal-peptide can be fused to the N-terminal of the gene of interest to be sorted to the plasma membrane in K. phaffii.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Rolli, Eleonora, et al. “GAS3, a Developmentally Regulated Gene, Encodes a Highly Mannosylated and Inactive Protein of the Gas Family of Saccharomyces Cerevisiae.” Yeast, vol. 27, no. 8, WILEY, 2010, pp. 597–610, doi:10.1002/yea.1788.

[2] Burgard, Jonas, et al. “The Secretome of Pichia Pastoris in Fed-Batch Cultivations Is Largely Independent of the Carbon Source but Changes Quantitatively over Cultivation Time.” Microbial Biotechnology, vol. 13, no. 2, John Wiley and Sons Ltd, 2020, pp. 479–94, doi:10.1111/1751-7915.13499.

[3] Almagro Armenteros, Jose Juan, et al. “DeepLoc: Prediction of Protein Subcellular Localization Using Deep Learning.” Bioinformatics, edited by John Hancock, vol. 33, no. 21, OXFORD UNIV PRESS, 2017, pp. 3387–95, doi:10.1093/bioinformatics/btx431.