Part:BBa_K3841008

Methanol regulated promoter AOX1

The AOX1 promoter region from Komagataella phaffii (previously referred to as Pichia pastoris) with 89% identity to Part:BBa_I764001. The AOX1 promoter (BBa_I764001) is a methanol-inducible promoter [1] commonly used in K. phaffii GS115 to control the expression [2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Ellis, S. B., et al. “Isolation of Alcohol Oxidase and Two Other Methanol Regulatable Genes from the Yeast Pichia Pastoris.” Molecular and Cellular Biology, vol. 5, no. 5, 'American Society for Microbiology', 1985, pp. 1111–21, doi:10.1128/mcb.5.5.1111-1121.1985.

[2] Vogl, Thomas, and Anton Glieder. “Regulation of Pichia Pastoris Promoters and Its Consequences for Protein Production.” New Biotechnology, vol. 30, no. 4, ELSEVIER SCIENCE BV, 2013, pp. 385–404, doi:10.1016/j.nbt.2012.11.010.