Regulatory

Part:BBa_K3841005

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-08-17)


FLD1 promoter

This sequence contains the inducible transcription promoter FLD1, useful for controlled gene expression which is actively enhanced in Komagataella phaffii (previously referred to Pichia pastoris) in the presence of methylamine or methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 143
    Illegal EcoRI site found at 476
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 143
    Illegal EcoRI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 143
    Illegal EcoRI site found at 476
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 143
    Illegal EcoRI site found at 476
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 143
    Illegal EcoRI site found at 476
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Ellis, S. B., et al. “Isolation of Alcohol Oxidase and Two Other Methanol Regulatable Genes from the Yeast Pichia Pastoris.†Molecular and Cellular Biology, vol. 5, no. 5, 'American Society for Microbiology', 1985, pp. 1111–21, doi:10.1128/mcb.5.5.1111-1121.1985.


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