Regulatory

Part:BBa_K3841000

Designed by: Emil �rsted Christensen   Group: iGEM21_DTU-Denmark   (2021-08-16)


GAP1 promoter

The GAP1 promoter is regulating the the structural gene for the general amino acid permease [1]. The sequence of this promoter originates from Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 243

References

[1] Stanbrough, Michael, and Boris Magasanik. “Two Transcription Factors, Gln3p and Nil1p, Use the Same GATAAG Sites to Activate the Expression of GAP1 of Saccharomyces Cerevisiae.” Journal of Bacteriology, vol. 178, no. 8, American Society for Microbiology, 1996, pp. 2465–68, doi:10.1128/jb.178.8.2465-2468.1996.


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