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Part:BBa_K3776010

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)


NSI This is Neutral Site I (NSI) of S.elongatus UTEX 2973. Neutral sites are sites in the chromosome commonly used as sites for gene manipulation in S.elongatus as disrupting these sites does not cause discernible phenotype(Rosato, 2010). Our team is creating a crRNA targeting NSI for CRISPR technology to edit the genome of S.elongatus 2973.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 604
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 791
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 604
    Illegal NgoMIV site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


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