Coding

Part:BBa_K3756004

Designed by: Chen Xi   Group: iGEM21_NJU-China   (2021-09-29)


CKLF1C19-Lamp2b

It can express the fusion protein CKLF1C19-Lamp2b, where CKLF1C19 is a CCR3-targeting peptide, so it can specifically target to the surface of TH2 cells. Lamp2b is an exosomal membrane protein. Thus, fusion protein CKLF1C19-Lamp2b was able to target TH2 cells by exosomes secreted by cells expressing this protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1111
    Illegal SpeI site found at 424
    Illegal PstI site found at 808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 424
    Illegal PstI site found at 808
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1111
    Illegal SpeI site found at 424
    Illegal PstI site found at 808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1111
    Illegal SpeI site found at 424
    Illegal PstI site found at 808
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None