Protein_Domain

Part:BBa_K3740004

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-08-26)


Translocationl domain of Pyocin S2

Description

Involves in the translocation across the outer membrane, we used it to construct chimeric bacteriocin SE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 732
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 531
    Illegal AgeI site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

Bacteriocin activity proteins have three distinct domains arranged from N to C terminus that include receptor, translocase, and nuclease (with immunity protein-binding) domains. The translocation domain are species specific and it involves in the translocation across the outer membrane.

References

[1] Gupta S, Bram E E, Weiss R. Genetically programmable pathogen sense and destroy. [J]. Acs Synthetic Biology, 2013, 2(12):715-723

[2] Michel-Briand Y, Baysse C. The pyocins of Pseudomonas aeruginosa. [J]. Biochimie, 2002, 84(5-6):499-510

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