Protein_Domain

Part:BBa_K3740003

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-08-25)


Nuclease domain of Colicin E3

Description

Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site, we used it to construct chimeric bacteriocin SE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

The nuclease domain inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site and kills the cell. The nuclease domain interacts with the immunity protein.

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Categories
Parameters
None