Part:BBa_K3739070

J23100-B0030-pelB-Ag43-160N-his-GFP-B0010-J23100-B0030-pelB-Ag43-160N-Tevp-B0010

Ag43 is a surface display protein and GFP can show the expression effciency of Ag43, Tevp can cut Ag43, leaving pelB as a signal peptide.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 3590
  Illegal NheI site found at 3613
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 118
  Illegal NgoMIV site found at 1076
  Illegal NgoMIV site found at 2300
  Illegal NgoMIV site found at 2810
  Illegal NgoMIV site found at 2831
  Illegal NgoMIV site found at 3701
  Illegal NgoMIV site found at 4539
  Illegal AgeI site found at 1826
  Illegal AgeI site found at 2570
  Illegal AgeI site found at 2984
  Illegal AgeI site found at 3226
  Illegal AgeI site found at 5289
  Illegal AgeI site found at 6033
  Illegal AgeI site found at 6447
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 206