Composite

Part:BBa_K3739062

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-10)


pDawn-B0030-GFP-rrnB T1

pDawn promoter system can be activated by blue light.


Biology and Usage

pDawn is a kind of regulation system that has been illustrated effectively in Vibrio natriegens which based on optogenetics. Under the irradiation of blue light, a series of regulatory elements will work on regulating the expression of genes that inserted into multiple cloning site In its downstream.

Gfp codes for a green fluorescence protein. The expression level of GFP can be used to characterize the effect of blue light induction in pDawn system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI.rc site found at 525


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