Coding

Part:BBa_K3715107

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-26)


PETase_MT3


PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well,PETase_MT3 is a complicated mutation of PETase, which contains 12 mutation sites: S54W-S92P-T113P-Q119D-Q127L-Q182I-S193R-S214F-K252L-T266P-N275F


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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