Composite

Part:BBa_K3482043

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-24)


azurin (UNIL) under pTetO1

Azurin protein under a pTet01 promoter

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.

We used this part for protein expression in a sponge plasmid for oscillatory expression with a repressilator


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 55
    Illegal NheI site found at 336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 55
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None