Regulatory

Part:BBa_K3482011

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)


pTac promoter

The pTac promoter sequence here is preceded by a binding region (operator) for the repressor LacI; the binding of LacI prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to LacI and releases it from its operator.


We use this pTac promoter in our kill switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None