Part:BBa_K3482000

heat-inducible RNA-thermosensor 2U

The 2U RNA thermosensor is inserted directly upstream of the gene of interest. The gene expression is then modulated by temperature.

Usage and Biology

RNA thermosensors are non-coding RNA molecules that are thermosensitive. When placed upstream of a gene, they can regulate the translation of this gene. RNA thermosensors usually act by changing in the secondary structure due to the change of temperature. These changes will block or free the access of the ribosome to the ribosome binding site (RBS) and thus regulate translation. Thermosensors are often involved in the regulation of heat-shock and cold-shock proteins.

We used this RNA thermosensors to regulate the expression of a toxin or an antitoxin in our kill switch

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482017 .

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 14
  Illegal PstI site found at 20
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 14
  Illegal PstI site found at 20
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 14
  Illegal PstI site found at 20
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 14
  Illegal PstI site found at 20
  
• 1000
  COMPATIBLE WITH RFC[1000]