Coding

Part:BBa_K3468385

Designed by: Xujia Liu   Group: iGEM20_TJUSLS_China   (2020-10-23)


PETase V134I

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from V to I at 134 position which can be more stable in higher temperature compared with the wild type.The DDG of this mutant is -1.0411.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None