Plasmid

Part:BBa_K3452894

Designed by: Haoran Hu   Group: iGEM20_CSU_CHINA   (2020-10-27)


pVZ322-hMT-1A

Plasmids that can be expressed in E. coli and Synechocystis sp. PCC 6803. It should be noted that the use of triparetal conjugation is required for expressing in PCC 6803


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9658
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
    Illegal PstI site found at 9646
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
    Illegal PstI site found at 9646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 862
    Illegal BglII site found at 1051
    Illegal BglII site found at 3520
    Illegal BamHI site found at 673
    Illegal XhoI site found at 2477
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9658
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
    Illegal PstI site found at 9646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9658
    Illegal SpeI site found at 946
    Illegal SpeI site found at 1135
    Illegal PstI site found at 1155
    Illegal PstI site found at 9646
    Illegal AgeI site found at 724
    Illegal AgeI site found at 892
    Illegal AgeI site found at 5024
    Illegal AgeI site found at 5425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9634


[edit]
Categories
Parameters
None