Plasmid_Backbone

Part:BBa_K3452893

Designed by: Haoran Hu   Group: iGEM20_CSU_CHINA   (2020-10-27)


pVZ322

Plasmids that can be expressed in E. coli and Synechocystis sp. PCC 6803. It should be noted that the use of triparetal conjugation is required for expressing in PCC 6803.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 8384
    Illegal PstI site found at 8372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 8372
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 2246
    Illegal XhoI site found at 1203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 8384
    Illegal PstI site found at 8372
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 8384
    Illegal PstI site found at 8372
    Illegal AgeI site found at 3750
    Illegal AgeI site found at 4151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 8360


[edit]
Categories
Parameters
None