Plasmid

Part:BBa_K3452889

Designed by: Haoran Hu   Group: iGEM20_CSU_CHINA   (2020-10-27)


pVZ322-TMCd1

Plasmids that can be expressed in E. coli and Synechocystis sp. PCC 6803. It should be noted that the use of triparetal conjugation is required for expressing in PCC 6803


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9445
    Illegal PstI site found at 9433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 9433
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3307
    Illegal BamHI site found at 495
    Illegal XhoI site found at 2264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9445
    Illegal PstI site found at 9433
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9445
    Illegal PstI site found at 9433
    Illegal AgeI site found at 4811
    Illegal AgeI site found at 5212
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9421


[edit]
Categories
Parameters
None