Plasmid

Part:BBa_K3452887

Designed by: Haoran Hu   Group: iGEM20_CSU_CHINA   (2020-10-27)


pVZ322-NRAMP5

Plasmids that can be expressed in E. coli and Synechocystis sp. PCC 6803. It should be noted that the use of triparetal conjugation is required for expressing in PCC 6803


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10042
    Illegal PstI site found at 10030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 10030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3904
    Illegal BamHI site found at 183
    Illegal XhoI site found at 2861
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10042
    Illegal PstI site found at 10030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10042
    Illegal PstI site found at 10030
    Illegal NgoMIV site found at 214
    Illegal NgoMIV site found at 1168
    Illegal AgeI site found at 673
    Illegal AgeI site found at 5408
    Illegal AgeI site found at 5809
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 10018


[edit]
Categories
Parameters
None