Plasmid

Part:BBa_K3452886

Designed by: Haoran Hu   Group: iGEM20_CSU_CHINA   (2020-10-27)


pVZ322-MntH

Plasmids that can be expressed in E. coli and Synechocystis sp. PCC 6803. It should be noted that the use of triparetal conjugation is required for expressing in PCC 6803


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9697
    Illegal PstI site found at 9685
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 9685
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3559
    Illegal XhoI site found at 2516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9697
    Illegal PstI site found at 9685
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9697
    Illegal PstI site found at 9685
    Illegal NgoMIV site found at 166
    Illegal NgoMIV site found at 508
    Illegal NgoMIV site found at 937
    Illegal AgeI site found at 5063
    Illegal AgeI site found at 5464
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9673


[edit]
Categories
Parameters
None