Plasmid

Part:BBa_K3452607

Designed by: Tianyuan Xie   Group: iGEM20_CSU_CHINA   (2020-10-27)


pVZ322-IRT1

Plasmids that can be expressed in E. coli and Synechocystis sp. PCC 6803. It should be noted that the use of triparetal conjugation is required for expressing in PCC 6803

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 8384
    Illegal PstI site found at 8372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 8372
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2246
    Illegal XhoI site found at 1203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 8384
    Illegal PstI site found at 8372
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 8384
    Illegal PstI site found at 8372
    Illegal NgoMIV site found at 9288
    Illegal AgeI site found at 3750
    Illegal AgeI site found at 4151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 8360


[edit]
Categories
Parameters
None