Composite

Part:BBa_K3396011

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-25)


GFP-Fluc


Usage and Biology

This is a GFP-coupled firefly luciferase, used as reporter system in the Dual Luciferase Reporter detecting method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 2187
    Illegal NgoMIV site found at 2208
    Illegal AgeI site found at 1911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI.rc site found at 2093


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Categories
Parameters
None