Coding

Part:BBa_K3389002

Designed by: M. Hammes, H. Sanchez   Group: iGEM20_SUNY_Oneonta   (2020-10-23)


RAD27 Flappase coding sequence

This part codes for RAD27 flap endonuclease 1 ("flappase") from Saccharomyces cerevisiae. RAD27, also known as FEN-1, is a structure-specific flap endonuclease that will cleave overlapping oligonucleotides at the site of a single target nucleotide in the context of a known sequence. One use for this is to distinguish between single nucleotide polymorphisms.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 273
    Illegal BglII site found at 666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


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Categories
Parameters
None