Part:BBa_K3385056

SigPuppy

About the part: This is the synthetic signal peptide SigPuppy, which was predicted by the model SignalPrepper. The model predicted this peptide to have high probability of being a strong signal peptide.

Functionality: This signal peptide was investigated in Deeploc [1] to predict the localization of the protein which it is inserted upstream of.

Localization predicted by Deeploc.

Result: A tissue PCR was performed to show that the synthetic signal peptide can be successfully integrated in the A. niger genome.

Tissue PCR showing the integration of the synthetic signal peptides SigPower, SigPeanut and SigPilot into the A. niger genome.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References:
[1] Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics, btx431