Part:BBa_K3385051

SigP_cbhB

About the part: This is the signal peptide for 1,4-beta-D-glucan cellobiohydrolase B (cbhB) in Aspergillus niger. The signal peptide was predicted from SignalP-5.0 [1].

Functionality: This signal peptide was investigated in Deeploc [2] to predict the localization of the protein which it is inserted upstream of.

Localization predicted by Deeploc.

Results: The efficiency of the signal peptide was tested through a starch/iodine plate assay, where a no colouring zone shows breakdown of starch. However, this testing is not conclusive as other amylases may be overexpressed. Therefore, this part needs further testing.

Starch/iodine assay showing the breakdown of starch by the ΔglaA and the ΔglaA with glucoamylase inserted with the cbhB signal peptide.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References:
[1] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)

[2] Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics, btx431