Measurement

Part:BBa_K3380690

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-25)


TXO PbrR regulated construct under BBa_K3380106 promoter expressing iSpinach

Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the PbrR transcription factor. Upon addition of the Pb, the transcription factor unbinds allowing the native Cupriavidus Metallidurans CH34 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.


Usage and Biology

Can be used in cell free biosensors

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None