Regulatory

Part:BBa_K3347017

Designed by: Jackson Duke Harris   Group: iGEM20_USAFA   (2020-10-16)


Improved prma promoter from Rhodococcus jostii RHA1

This promoter is an improved variant of a previous part: BBa_K2911000 from the 2019 USAFA iGEM team. In the previous version part, our design method created single nucleotide polymorphisms in critical regions of the promoter, lending to the issue of failed or significantly decreased expression in the reporter gene. To combat this, the sequence was directly amplified from the source genome in Rhodococcus jostii RHA1 with the use of specifically designed primers that would incorporate standard igem cut sites for use in RFC10 assembly.
This part was used as the functional regulator in our PFAS biosensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 150
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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