Composite

Part:BBa_K3338021

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


SynthP_1-hGLuc

The presented composite part consists of hGLuc under the control of the synthetic promoter_1 with NF-κ and AP1 binding sites. The part can be used to examine the basal activity and the LPS sensitivity of the promoter. As a control CMV-hGLuc (BBa_K3338017) can be used.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NotI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

This part was used to assess the LPS sensitivity of the “Synthetic promoter_1 with NF-κB and AP1 binding sites”. It could be shown that the promoter is strongly induced following LPS-treatment of the transfected cells. However, the LPS-sensitivity of Synthetic promoter_2 (BBa_K3338002) is even higher. Comparing its basal activity with other promoters revealed an approximately 25-fold higher activity than the natural CMV promoter. A detailed documentation can be found on the page of the basic part BBa_K3338007.


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Parameters
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