Composite

Part:BBa_K3338018

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


CMV-MagA-FLAG

MagA from Magnetospirillum magnetotacticum expressed with C-terminal Flag-tag for expression in mammalian cells under control of the CMV-enhancer/promoter. The part can be used to confirm MagA expression without the relatively large EGFP-tag with a Western Blot using a FLAG-tag specific antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1836
    Illegal BsaI.rc site found at 1168
    Illegal BsaI.rc site found at 1717
    Illegal SapI site found at 858


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Categories
Parameters
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