Coding

Part:BBa_K3191111

Designed by: Logan Hauder   Group: iGEM19_UNebraska-Lincoln   (2019-10-16)


AgrA

AgrA is a response regulator protein native to Staphylococcus aureus. It is a component of the accessory gene regulator (agr) system, which is responsible for quorum sensing in response to an autoinducing peptide (AIP). The AgrA protein is phosphorylated by the membrane protein AgrC, which then activates transcription of genes under the influence of P1, P2, and P3 promoters.

This part modifies the sequence of a subunit in an existing BioBrick, BBa_I746100. The AgrA sequence upon which BBa_I746100 was based differs from the UniProt sequence for AgrA, missing the first 30 amino acids. This AgrA sequence includes these 30 amino acids at the N-terminus of the peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 373
    Illegal BsaI.rc site found at 421


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Categories
Parameters
None