Coding

Part:BBa_K3191110

Designed by: Logan Hauder   Group: iGEM19_UNebraska-Lincoln   (2019-10-16)


AgrC

AgrC is a membrane-bound protein native to Staphylococcus aureus. It is a component of the accessory gene regulator (agr) system, which is responsible for quorum sensing in response to an autoinducing peptide (AIP). The AgrC protein interacts with AIP, triggering an agr-dependent response through phosphorylation of the next intermediate, AgrA.

This part modifies the sequence of a subunit in an existing BioBrick, BBa_I746100. The AgrC sequence upon which BBa_I746100 was based differs from the UniProt sequence for AgrC, missing the first 16 amino acids. This AgrC sequence includes these 16 amino acids at the N-terminus of the peptide.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None